Date/Time
Date(s) - 28/11/2012
3:20 pm - 4:20 pm

Title: Biophysics of Lipid Bilayer and Protein Interaction Modulated by Curvature

Speaker: Dr. Tobias Baumgart

Institute: University of Pennsylvania

Location: ABB 102

Description:

Membrane curvature has recently emerged at the forefront of membrane biophysics. Numerous proteins involved in membrane curvature sensing and membrane curvature generation have recently been discovered, including proteins containing the crescent-shaped BAR domain as membrane binding and shaping module. Accordingly, the structure determination of these proteins and their multimeric complexes is increasingly well-understood. Substantially less understood, however, are thermodynamic and kinetic aspects and the detailed mechanisms of how these proteins interact with membranes in a curvature-dependent manner. New experimental approaches need to be combined with established techniques to be able to fill in these missing details. Here we use model membrane systems in combination with a variety of biophysical techniques to characterize mechanistic aspects of BAR domain protein function. We first establish kinetic and thermodynamic aspects of BAR protein dimerization in solution, and then investigate kinetic aspects of membrane binding. We identify significantly different time scales for protein dimerization, membrane association, membrane insertion, and oligomerization and investigate numerous additional aspects of curvature influencing aspects of membrane biophysics